Essential 6™ Medium

Brand: Thermo Fisher Scientific

Essential 6 Medium is a feeder-free and xeno-free medium that supports the reprogramming of somatic cells and the spontaneous or directed differentiation of human pluripotent stem cells (PSCs).

In addition, Essential 6 Medium can be used as a base for custom media for the culture of PSCs.

The formulation is based on a medium originally developed by Guokai Chen et. al. (1) in the laboratory of James Thomson and published as 'E6.'

With only six essential components, Essential 6 Medium helps minimize variability.
Features
  • Differentiation - does not contain bFGF, which inhibits differentiation
  • Reprogramming - does not contain TGFβ, which has a negative effect on reprogramming efficiency
  • Flexibility - provides a flexible format where the levels of TGFβ and bFGF can be adjusted and additional components can be added to match a given application

Specifications

Species

  • Human

Classification

  • Xeno-Free

Manufacturing Quality

  • cGMP for Medical Devices, 21 CFR Part 820 and ISO 13485

Serum Level

  • Serum-Free

Volume (Metric)

  • 500 ml

Form

  • Liquid

Cell Type

  • Stem Cells (IPS - Induced Pluripotent Stem)

Culture Type

  • Feeder Cell-Free

Storage

  • Store at 2 to 8° C and Protect from light

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