Essential 6 Medium is a feeder-free and xeno-free medium that supports the reprogramming of somatic cells and the spontaneous or directed differentiation of human pluripotent stem cells (PSCs).
In addition, Essential 6 Medium can be used as a base for custom media for the culture of PSCs.
The formulation is based on a medium originally developed by Guokai Chen et. al. (1) in the laboratory of James Thomson and published as 'E6.'
With only six essential components, Essential 6 Medium helps minimize variability.
Features- Differentiation - does not contain bFGF, which inhibits differentiation
- Reprogramming - does not contain TGFβ, which has a negative effect on reprogramming efficiency
- Flexibility - provides a flexible format where the levels of TGFβ and bFGF can be adjusted and additional components can be added to match a given application