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Active Ras Pull-Down and Detection Kit
Brand: Thermo Fisher ScientificThe Thermo Scientific Active Ras Pull-Down and Detection Kit enables selective enrichment and detection of GTP-bound Ras GTPase through a specific protein interaction with the Raf1 protein-binding domain.
The Active Ras Pull-Down and Detection Kit includes purified GST-Raf1 Ras-binding domain (RBD), glutathione agarose resin, positive and negative controls (GTPγS and GDP, respectively), lysis/binding/washing buffer, anti-Ras antibody, SDS sample buffer, spin columns and collection tubes.
The kit was validated using lysates from NIH 3T3 cells, a cell line known to have robust Ras activity.
Features- Highly sensitive and accurate - optimized reagents, specific anti-Ras antibody and Western blot procedure ensure accurate controls and semi-quantitative results
- Validated - functionally tested for Ras detection to ensure quality and performance
- Compatible - effective for a variety of cell types from mouse, rat and human sources
Specifications
Assay
- Protein Quantitation Assay
Substrate Properties
- Protein-based Substrate
Substrate Type
- GTPase Substrate
Target
- GTPases
- ATPases
- Phosphodiesterases
- Kinases (Multiple)
- Phosphatases
Contents & Storage
- Lysis/Binding/Wash Buffer (1X), 100 mL
- SDS Sample Buffer (2X), 1.5 mL
- GTPyS (100X), 50 µL
- GDP (100X), 50 µL
- Glutathione Agarose Resin, 3 mL
- Anti-Ras Antibody, 5 units, 250 µL
- Spin Cups, 30 cups
- GST-Raf1-RBD Fusion Protein, 1 vial
- Collection Tubes, 90 Tubes