Pierce™ BCA Protein Assay Kit

Brand: Thermo Fisher Scientific

Pierce™ BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible protein assay for determination of protein concentration.

Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.

The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications.

Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.
  • Colorimetric - estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
  • Uniformity - exhibits less protein-to-protein variation than dye-binding methods (Bradford)
  • Compatibility - unaffected by typical concentrations of most ionic and non-ionic detergents
  • Assay time - 30-min incubation; much easier and four times faster than classical Lowry methods
  • Assay range - linear working range for BSA of 20 to 2000 µg/mL
  • Sensitivity - detect down to 5 µg/mL with enhanced protocol


For Use with

  • Spectrophotometer
  • Microplate Reader


  • Protein Quantitation


  • 1 Liter Kit

Detection Method

  • Colorimetric

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